Journal: Brain : a journal of neurology

Article Title: Amyloid-β dimers in the absence of plaque pathology impair learning and synaptic plasticity.

doi: 10.1093/brain/awv355

Figure Lengend Snippet: Figure 3 Pathological consequences in tgDimer mice. (A) Absence of hyperphosphorylated endogenous tau in tgDimer mice. Top: Western blots of 30 mg of crude brain homogenates from 13-month-old tgDimer or wild-type C57BL/6N mice stained with antibodies recognizing endogenous total tau (i, HT7) or tau that was phosphorylated at Ser202 and Thr205 (ii, AT8). Detection of actin was used as internal control (bottom). Densitometric analysis of tau signals were normalized to actin [mean + SEM; n = 8 (C57BL/6N), n = 12 (tgDimer)]. (B and C) Absence of neuroinflammation in tgDimer mice. Sagittal brain sections of adult (9 months) or old (24 months) tgDimer and C57BL/6 N mice were stained with markers for astroglia (GFAP) or microglia (Iba1). Cortical and hippocampal regions are shown. In comparison to age-matched wild-type mice no increased neuroinflammation was observed in tgDimer mice (n = 3, Scale bars = 500 mm). Brain sections of 12-month-old CRND8 mice served as controls (right).

Article Snippet: 6F/3D, which is specific for amino acids 9–14 from amyloid-b (M0872) and a GFAP-specific antibody (Z0334) were purchased from Dako, an anti-Iba1 antibody (AP08912PU-N) was bought from Acris Antibodies and the total tau antibody HT7 (MN1000), as well as the anti-phospho-PHF-tau antibodies AT8 (MN1020) and AT180 (MN1040), from Thermo Scientific.

Techniques: Western Blot, Staining, Control, Comparison

Journal: Mediators of Inflammation

Article Title: Lipopolysaccharide Sensitizes Steroid-Induced Brain Injury in Neonatal Rat Pups

doi: 10.1155/mi/8285898

Figure Lengend Snippet: Determination of TUNEL-positive cells. Sections from three brain regions (hippocampus, cerebellum, and brain stem) were stained using NeuN, GFAP, Iba1, and SOX10 to identify neurons, astrocytes, microglia, and oligodendrocyte precursor cells, respectively in TUNEL + cells. (A–D) Representative immunofluorescent micrographs of dual NeuN + /TUNEL + (A), GFAP + /TUNEL + (B), Iba1 + /TUNEL + (C), and SOX10 + /TUNEL + (D) cells. White arrows indicate dual positive stained cells. DAPI staining was used to confirm the presence of cell nuclei. (E–G) Quantitative results on TUNEL-positive cell types presented in pie charts for three different brain regions.

Article Snippet: After TUNEL stain, brain sections blocked with 3% normal goat serum (Cat# S26-M, Sigma-Aldrich, St. Louis, MO) were prepared in phosphate-buffered sal with Tween 20 (PBST) for 1 h at room temperature and probed with one of the following primary antibodies: mouse antineuronal nuclear antigen (NeuN) (1:250 dilution, Cat. # MAB377, Merck KGaA), rabbit antiglial fibrillary acidic protein (GFAP) (1:250 dilution, Cat. # Z0334, Dako-Agilent, Santa Clara, CA), rabbit anti-ionized calcium-binding adapter molecule-1 (Iba1) (1:250, Cat. #: 019-19741, Wako Pure Chemical Industries, Osaka, Japan), and rabbit anti-SRY-box transcription factor 10 (SOX10) (1:250 dilution, Cat. #: TA381887, Ori-Gene, Rockville, MA) for 16 h at room temperature.

Techniques: TUNEL Assay, Staining